Phylogenetic analysis classified Xyn5A in subfamily 35 of GH family 5, a subfamily of unknown function. Four enzymes, Xyn5A, Xyn10B, Xyn11A, and Xyn30A, were successfully identified, which belong to glycoside hydrolase (GH) families, 5, 10, 11, and 30, respectively. Enzymes were purified from supernatant of culture broth of strain H2C based on solubilizing activities toward CX. Analysis based on the 16S rRNA sequence placed strain H2C within genus Paenibacillus. ![]() To find enzymes capable of hydrolyzing CX, we isolated a bacterial strain (named H2C) from soil, by enrichment culture using non-starch polysaccharides of corn as the sole carbon source. Corn fibre xylan (CX) shows high resistance to enzymatic hydrolysis due to its densely decorated side chains.
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